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Fig. 6 | Journal of Biological Engineering

Fig. 6

From: CRISPR/Cas9 mediated T7 RNA polymerase gene knock-in in E. coli BW25113 makes T7 expression system work efficiently

Fig. 6

Confirmation of the efficiency of the T7 expression system in BW25113-T7. (A) Expression of sYFP under control of T7-LacI promoter in different bacterial strains. The fluorescent signal was detected by Multimode Reader (read: 503, 540) and was normalized to the cell density (OD600). (B) The biosynthesis of ALA (5-Aminolevulinic Acid) by T7 Expression System in different bacterial strains. These strains were cultured in Medium M9YE. All experiments were performed in triplicates. P values were calculated using Tukey’s multiple comparisons test (*P < 0.05, **P < 0.01, ***P < 0.001)               

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