Table 2 Primer pairs for CRISPR/Cas9 induced Knock-In
Primer | Sequences (5'to3') | Base Number | Note |
|---|---|---|---|
Primer F1 | gcttgttggtacgcttctgccattcatccgcttattatcac | 41 | Positions in boldface type are homologous fragments designed for Takara In-Fusion |
Primer R1 | accgcggcgtgggtacctggcgttacccaacttaat | 36 | |
Primer ybhC-F | tacccacgccgcggttattg | 20 | Â |
Primer ybhC-R | aagcgtaccaacaagcgcca | 20 | Â |
Primer F2 | cttttcgtgcgcgcataacgttgttaatctgtacctggtc | 40 | Positions in boldface type are homologous fragments designed for Takara In-Fusion |
Primer R2 | tggtgtccgggatctgtatcgtttctggtcgcggcg | 36 | |
Primer RNAP-F | cagatcccggacaccatcga | 20 | Â |
Primer RNAP-R | atgcgcgcacgaaaagcatc | 20 | Â |
Primer Donor-F | tacccacgccgcggttattg | 20 | Â |
Primer Donor-R | aagcgtaccaacaagcgcca | 20 | Â |
Primer N20-F | gttttagagctagaaatagcaagttaaaat | 30 | Embellished by 5’-phosphorylation |
Primer N20-R | tctggaaacgaatcgtcagcactagtattatacctaggac | 40 |