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Table 4 ALA production in different bacterial strains expressing various related genes

From: CRISPR/Cas9 mediated T7 RNA polymerase gene knock-in in E. coli BW25113 makes T7 expression system work efficiently

Strains

Plasmid

Expressed genes

ALA accumulation (mg/L)

BW25113

pET28b-ALA-LA

hemA, hemL

2.9 ± 0.9

BW25113-T7

pET28b-ALA-LA

hemA, hemL

165.8 ± 2.7

BL21(DE3)

pET28b-ALA-LA

hemA, hemL

163.3 ± 2.6

BW25113

pET28b-ALA-LAR

hemA, hemL, RhtA

2.4 ± 0.3

BW25113-T7

pET28b-ALA-LAR

hemA, hemL, RhtA

626.3 ± 32.8

BL21(DE3)

pET28b-ALA-LAR

hemA, hemL, RhtA

891.3 ± 22.3

  1. The results are the average of three individual experiments