Skip to main content
Fig. 1 | Journal of Biological Engineering

Fig. 1

From: Structural design of tetravalent T-cell engaging bispecific antibodies: improve developability by engineering disulfide bonds

Fig. 1

Design of single additional disulfide bond in CH1/CL domain of tetravalent BsAbs against glypican-3 and CD3 and its expression. (A) The schematic construction of tetravalent BsAb and crystal structure of CH1 and CL domain that are colored by yellow and gray. green: native disulfide bridges; red: engineered disulfide bridges. (B) Western blot (WB) of BsAbs expression. Blank: cell supernatant of a blank vector. Determination of monomeric BsAb (C) and expression levels (D). Mean ± SD (n = 3), *P < 0.05

Back to article page

Journal of Biological Engineering

ISSN: 1754-1611