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Fig. 2 | Journal of Biological Engineering

Fig. 2

From: Structural design of tetravalent T-cell engaging bispecific antibodies: improve developability by engineering disulfide bonds

Fig. 2

Single or double disulfide bonds introduction in the Fab domain of tetravalent BsAbs against glypican-3 and CD3. (A) Schematic of four BsAbs with introduced zero, one or two interdomain disulfide bonds in Fab region. The residues in CH1126/CL121 and VH44/VL100 colored by red were mutated to cysteines. (B, C) Expression and (D) purified yield levels of four BsAbs. Mean ± SD (n = 3), *P < 0.05

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