Fig. 5

Inhibition of cell growth by the BsAbs targeting glypican − 3 and CD3. The four BsAbs were cultured with SK-GPC3 cells or SK-Hep-1 cells (target cells, T) and PBMCs (effector cells, E) at a E:T ratio of 10:1 for 48 h. (A) Inhibition of proliferation was measured by CCK-8 assay. Data analysis of IC50 (B) and Maximum efficacy (C) performed by Prism Version 8.0. Mean ± SD (n = 3), *P < 0.05, **P < 0.01. SK-GPC3 cells: glypican-3 positive; SK-Hep-1 cells: glypican-3 negative