Table 1 Thermostability and affinity of BsAbs with single disulfide bond introduction. The T_m values of BsAbs were obtained from a single determination, and the values of affinity were shown as mean ± SD (n = 3)

Thermostability (T_m, ℃)

67.6

68.7

68.4

67.8

67.8

68.2

67.3

67.8

Affinity to Glypican-3 (nM)

1. 9 ± 0.1

2.4 ± 0.1

3.2 ± 0.3

2.2 ± 0.1

2.6 ± 0.2

4.5 ± 0.4

6.6 ± 0.2

2.8 ± 0.1

Affinity to CD3 (nM)

48.4 ± 6.2

42.1 ± 3.6

52.9 ± 5.4

85.3 ± 7.0

68.9 ± 3.2

170.2 ± 23

88.8 ± 6.5

94.0 ± 9.2