Fig. 3
From: AZD2014, a dual mTOR inhibitor, attenuates cardiac hypertrophy in vitro and in vivo
Comparison of AZD2014 (100 nM) and Rapamycin (100 nM) on phenylephrine (PE)-induced cardiac hypertrophy. a The protein expression levels of Myh6 and ANP in cardiomyocytes treated as indicated were determined by FACS. b Representative fluorescence microscopy images of immunocytochemistry for Myh6 and ANP of cardiomyocytes treated as indicated. Scale bar = 25 μm. c Cell area and nucleus size in cardiomyocytes treated as indicated were determined by fluorescence microscopy and Image J. Graphic representation of cell area and nuclear size from 20 randomly selected measured cells. #; p > 0.05, *; p < 0.05 vs. Control (non-PE treatment). Scale bar = 25 μm