Fig. 4
Cell culture parameters during seeding, static cultivation and dynamic culture. Parameters during static culture: Supervision of pH during static cultivation every three hours revealed the values gradually decreased (A). While pO2 values maintained stable (B), pCO2 values significantly rose (C). Glucose (D) and lactate (E) values respectively decremented and incremented significantly during the static cultivation, while Na+ (F) and K+ (G) concentrations preserved stability throughout its duration. (****, p < 0.0001). Parameters during dynamic cultivation: pH which preserved stability (H), pO2, which significantly incremented presumably due to increasing flow rates (I) and pCO2 (J), glucose (K), lactate (L), Na+ (M) and K+ (N) which also preserved stability throughout the whole duration of the dynamic culture. (****, p < 0.0001). The dots represent different experiments