Fig. 7

Histological and immunohistochemical evaluation of seeded vascular grafts after perfusion with whole blood. Histological evaluation of the whole blood perfused seeded grafts revealed findings similar to the seeded, non-blood perfused grafts, whereby cells were attached to the basal membrane (A). Staining with anti-CD31 (B), anti-CD34 (C), anti-eNOS (D) and anti-vWF antibody (E) revealed the majority of the cells were endothelial cells, while staining with anti-CD90 antibody (F) revealed the location of the hMSC. Scale bar represents 50 μm