Fig. 3
From: Knockdown of NCK1-AS1 inhibits the development of atherosclerosis by targeting miR-1197/COX10 axis
NCK1-AS1 regulated COX10 by sponging miR-1197. A Correlation between NCK1-AS1 and miR-135a-5p level in AS blood samples. B Schematic model of miR-1197 binding site within NCK1-AS1 predicted by Starbase and the construction of mutant miR-1197 binding site. C VSMCs were transfected with miR-1197 mimics or inhibitor, and qRT-PCR for miR-1197 level. D VSMCs were transfected with miR-1197 mimics or mimics NC, and the relative luciferase activity. E Schematic model of miR-1197 binding site within 3′UTR COX10 predicted by Targetscan and the construction of mutant miR-1197 binding site. F VSMCs were transfected with miR-1197 mimics or mimics NC, and the relative luciferase activity. G - I VSMCs were transfected with sh-NCK1-AS1, and then treated with 50 μg/ml ox-LDL for 24 h. QRT-PCR for miR-1197 level G, COX10 level H, and western blot for COX10 level I. * p < 0.05, ** p < 0.01, and *** p < 0.001