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Fig. 4 | Journal of Biological Engineering

Fig. 4

From: Knockdown of NCK1-AS1 inhibits the development of atherosclerosis by targeting miR-1197/COX10 axis

Fig. 4

Knockdown of NCK1-AS1 attenuated ox-LDL-induced VSMCs injury through regulating miR-1197/COX10 axis. A and B CCK-8 assay for cell viability A and flow cytometry for cell apoptosis B after co-transfection of sh-NCK1-AS1 and miR-1197 inhibitor in VSMCs followed by 50 μg/ml ox-LDL for 24 h. C and D CCK-8 assay for cell viability C and flow cytometry for cell apoptosis D after co-transfection of sh-NCK1-AS1 and pc-COX10 (COX10 overexpressing plasmid) in VSMCs followed by 50 μg/ml ox-LDL for 24 h. E and F VSMCs were co-transfected with sh-NCK1-AS1 and miR-1197 inhibitor E, or sh-NCK1-AS1 and pc-COX10 F, and then treated by 50 μg/ml ox-LDL for 24 h. The MDA content was measured by specific detection kit. * p < 0.05, ** p < 0.01, and *** p < 0.001

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