Table 2 The sequences of specific primers
From: Knockdown of NCK1-AS1 inhibits the development of atherosclerosis by targeting miR-1197/COX10 axis
Gene name | Primer sequence (5′ to3’) |
|---|---|
NCK1-AS1 | Forward: 5′-AGTTCAGCCCCCACTGCTCT-3′ Reverse: 5′-TGGTTTGAGTTCCCATTTCTC-3’ |
COX10 | Forward: 5’-TCTGTTGTGGCTGGCTTTGGAC-3′ Reverse: 5′-CTTCTCTGGCAATTCTTTCCTGG-3’ |
IL-1β | Forward: 5’-CCACAGACCTTCCAGGAGAATG-3′ Reverse: 5′-GTGCAGTTCAGTGATCGTACAGG-3’ |
IL-6 | Forward: 5’-AGACAGCCACTCACCTCTTCAG-3′ Reverse: 5′-TTCTGCCAGTGCCTCTTTGCTG-3’ |
TNF-a | Forward: 5’-CTCTTCTGCCTGCTGCACTTTG-3′ Reverse: 5′-ATGGGCTACAGGCTTGTCACTC-3’ |
GAPDH | Forward: 5’-ATCCACGGGAGAGCGACAT-3′ Reverse: 5′-CAGCTGCTTGTAAAGTGGAC-3’ |
U6 | Forward: 5’-ACAGATCTGTCGGTGTGGCAC-3′ Reverse: 5′-GGCCCCGGATTATCCGACATTC-3’ |