Table 4 Advantages and Disadvantages of terminal sterilization methods
From: Application of decellularized bone matrix as a bioscaffold in bone tissue engineering
Sterilization techniques | Advantages | Disadvantages | References |
|---|---|---|---|
Gamma Irradiation | Fast Safe | Alters the mechanical strength of bioscaffolds | [81] |
Electron Beam Irradiation | High biocompatible Fast Safe | Alters the mechanical strength of bioscaffolds Damages the ECM architecture | [80] |
Ethylene Oxide | Has no effect on the ECM ‘s ability to bind T- cells. Has no effect on the secretion of growth factors on fibroblasts. | It alters the structure of the protein, making it impossible to trace these molecules. Changes the mechanical stability of the ECM. Mutagenic Carcinogenic It may promote immune response | [82] |
Supercritical Carbon Dioxide (principal method for terminal sterilization) | A practical method High biocompatible keeping the mechanical properties Preservation of scaffold stability Safe | No disadvantages have been reported for this method |