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Fig. 1 | Journal of Biological Engineering

Fig. 1

From: Organotypic whole hemisphere brain slice models to study the effects of donor age and oxygen-glucose-deprivation on the extracellular properties of cortical and striatal tissue

Fig. 1

Schematic of OWH slice culture methodology and experimental workflow. A Left: Coronal slices containing cortex (CTX) and striatum (STR) are plated onto PTFE membrane inserts. Right: a single OWH brain slice after explantation. Although not imaged in this study, hippocampal (HIP) and corpus callosum (CC) regions are also labeled for reference. Scale bars = 15 cm and 5 cm. B Experimental workflow. Slices from postnatal (P) day 10 or P17 aged rat donors are cultured up to 14 days, performing OGD on 4 DIV with endpoints at 5 days in vitro (DIV) (24 h post-OGD), 7 DIV (72 h post-OGD), and 10 DIV

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Journal of Biological Engineering

ISSN: 1754-1611