Fig. 5

Metabolic activity, LDH release, and cell proliferation profiles following OGD. A Metabolic activity of P10 slices after 1 h, 2 h, or 3 h OGD. Error bars represent median and IQR. B LDH release (%) profiles for P10 slices after 1 h, 2 h, or 3 h OGD. Error bars represent mean ± SD. C Metabolic activity profiles for P17 slices after 1 h, 2 h, or 3 h OGD. Error bars represent median and IQR. * denotes significant differences (p < 0.05) between all OGD groups (1 h, 2 h, and 3 h) and the NC. D LDH release (%) profiles for P17 slices after 1 h, 2 h, or 3 h OGD. Error bars represent mean ± SD. * denotes significant differences (p < 0.05) between all OGD groups (1 h, 2 h, and 3 h) and the NC. ☨ denotes significant differences (p < 0.05) between the 1 h and 3 h OGD groups and the NC. For (A) and (C), n = 6–12 slices per condition. For (B) and (D), n = 6 slices for all OGD conditions, and n = 12–42 slices for NC conditions. Each data point represents a single slice. E Percentage of proliferating cells in P10 slices throughout culture and after 2 h OGD. 24 h time points refer to time after OGD, equivalent to 5DIV; 72 h time points refer to 72 h after OGD, equivalent to 7DIV. Data from a NC slice at 144 h (10DIV) is provided for comparison. F Percentage of proliferating cells in the cortex and striatum following 2 h OGD and compared to NC. Each data point represents a single image from 3 total slices per group