Fig. 2From: Cloning, expression, and one-step purification/immobilization of two carbohydrate-binding module-tagged alcohol dehydrogenasesE. coli M15ΔglyA fed-batch cultures. Batch, fed-batch and induction ([IPTG] 0.25 mM) phases indicated. Culture conditions: 37 °C, pH 7.0, 450-1200 rpm, 60% PO2, 0.25 mM IPTG. A: His-ScADH, B: CBM3-ScADH and C: CBM9-ScADH. Arrows indicate the stop of the feeding (↓) and the resume of the feeding (↑)Back to article page