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Fig. 7 | Journal of Biological Engineering

Fig. 7

From: Cloning, expression, and one-step purification/immobilization of two carbohydrate-binding module-tagged alcohol dehydrogenases

Fig. 7

SDS–PAGE of FPLC affinity purifications of CBM9–ScADH on a 10 mL RAC column. Lanes M: molecular weight standard (kDa); lanes 1, 5 and 9: clarified cell extract prior to column loading; lanes 2, 6 and 10: column flow through; lanes 3, 7 and 11: column wash; lanes 4, 8 and 12: purified CBM9–ScADH eluted with 50 mM Tris-HCl buffer containing 2 M glucose. CBM9-ScADH (64 kDa) corresponding band indicated with arrow

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Journal of Biological Engineering

ISSN: 1754-1611