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Table 1 List of oligonucleotides used as primers in polymerase chain reactions of histidine-fused enzymes

From: Cloning, expression, and one-step purification/immobilization of two carbohydrate-binding module-tagged alcohol dehydrogenases

Name (sense)

5′ to 3′ DNA sequence

Size (bp)

Tm (°C)

GC (%)

his-ADH (Fw)

AGGAGAAATTAACCCATGGGCAGCAGCCATCAT

33

68

48

ScADH (Rev)

CTAATTAAGCTTCCCTTATTTAGAAGTGTCAACAACG

37

61

35

CBM3-linker (Fw)

AGGAGAAATTAACCCATGAACCTGAAAGTGGAA

33

63

39

CBM9-linker (Fw)

AGGAGAAATTAACCCATGGTGGCGACCG

28

66

54

Linker-ScADH (Rev)

GGATAGACATGCCGCTGCCGCT

22

66

64

Linker-ScADH (Fw)

CGGCAGCGGCATGTCTATCCCAGAAACTCA

30

68

57