Table 2 Production parameters of ScADH using E. coli M15ΔglyA; comparison between the three different N-terminal-fused tags

Enzyme activity (AU·L⁻¹)

2.83·10⁵

1.02·10⁵

1.16·10⁵

Enzyme titer (mg·L⁻¹)

1940

1550

1780

Volumetric productivity (AU·L⁻¹·h^− 1)

7870

2300

2520

Volumetric productivity (mg·L⁻¹·h^− 1)

54

35.3

38.6

Specific production (mg·g⁻¹DCW)

38.4

50.7

36.6

Specific production (AU·g⁻¹DCW)

5600

3320

2390

Specific activity (AU·mg⁻¹)

145.7

65.4

65.3

Specific activity (AU·μmol⁻¹) *

5380

3580

3860