From: Cloning, expression, and one-step purification/immobilization of two carbohydrate-binding module-tagged alcohol dehydrogenases
Tag
Total protein offered (mg)
Enzyme offered (mg)
Enzyme offered (AU)
RA
(%)
IY
Recovered activity (%)
CBM3
2.54 ± 0.04
0.37 ± 0.02
29 ± 0.5
86.1 ± 2.2
98.4 ± 1.3
73.6 ± 3.3
CBM9
2.37 ± 0.3
0.22 ± 0
14.2 ± 4.5
97.7 ± 1.7
99.7 ± 0.1
92.4 ± 3.9