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Table 3 Immobilization parameters of CBM-fused ScADH proteins onto RAC support

From: Cloning, expression, and one-step purification/immobilization of two carbohydrate-binding module-tagged alcohol dehydrogenases

Tag

Total protein offered (mg)

Enzyme offered (mg)

Enzyme offered (AU)

RA

(%)

IY

(%)

Recovered activity (%)

CBM3

2.54 ± 0.04

0.37 ± 0.02

29 ± 0.5

86.1 ± 2.2

98.4 ± 1.3

73.6 ± 3.3

CBM9

2.37 ± 0.3

0.22 ± 0

14.2 ± 4.5

97.7 ± 1.7

99.7 ± 0.1

92.4 ± 3.9