Table 4 FPLC-based ScADH purification results for CBM9-ScADH (top) and CBM3-ScADH (bottom)
Tag | Sample | Total activity (AU) | Total protein (mg) | Recovered activity (%) | Specific activity (AU·mg− 1) | Purification factor |
|---|---|---|---|---|---|---|
CBM9 | Lysate | 4117 ± 99 | 380.6 ± 8.8 | – | 10.8 ± 0.5 | – |
Eluted | 3813 ± 129 | 35.8 ± 2 | 94.7 ± 2.3 | 107.8 ± 6 | 10.1 | |
CBM3 | Lysate | 5850 ± 316 | 440 ± 23 | – | 13.3 ± 0.4 | – |
Eluted | 350 ± 1.2 | 8.8 ± 0.5 | 6 ± 0 | 39.7 ± 1.2 | 3 |