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Fig. 1 | Journal of Biological Engineering

Fig. 1

From: A tailored bioactive 3D porous poly(lactic-acid)-exosome scaffold with osteo-immunomodulatory and osteogenic differentiation properties

Fig. 1

Characterization of hBMSCs-derived exosomes. A Morphological characterization of MSCs-derived exosomes with uranyl acetate negative staining. Isolated exosomes exhibited a cup-shaped morphology. B Western blot analysis of exosomal surface marker. Exosomes were found to be enriched for the exosomal surface marker CD63. C Flow cytometry analysis of exosome internalization into RAW264.7 cells. D The mean fluorescence intensity (MFI) obtained from RAW264.7 cells treated with or without MSC-Exo. E Internalization of MSC-Exo into hBSMCs. Cellular internalization of MSC-Exo by flow cytometric analysis. F MFI quantification of hBMSCs after 24 h of incubation with isolated exosomes. G Uptake of exosomes released from MSC-Exo by hBMSCs. Exosomes were prelabeled with PKH26. The internalization of the exosomes was evaluated by a confocal laser scanning microscopy

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