Fig. 1

Identification of primary BMSCs from mice and the expressions of HSP70 in BMSCs after heat shock treatment. A Light microscopy images of bone marrow mesenchymal stem cells (BMSCs) cultured for 3 days (left) and 7 days (right). B Positive expressions of CD44, CD105, and negative expressions of CD34, CD45 were detected with flow cytometry. C CCK-8 was used to measure the cell viability of BMSCs with or without heat shock precondition. D The relative expression of HSP70 was assayed in heat shock preconditioned BMSCs with RT-PCR. Western blotting was used to measure the expressions of HSP70 from heat shock preconditioned BMSCs (E). The data were shown with mean ± SD. n = 3 for each group. ***p < 0.001 compared to control. Mann Whitney test