Fig. 3
From: Label-free detection of leukemic myeloblasts in hyaluronic acid
Hyaluronic acid makes cellular trails of K562 clearly visible. (A) K562 and leukocytes were treated with 0.01, 0.02, 0.05, or 0.1% of hyaluronic acid for 12 h and cell viability was measured with CCK-8 kit. (B) Viscosity of hyaluronic acid was measured with a cone-and-plate digital viscometer at six different shear rates. The viscosity values for 0.01, 0.02, 0.05% hyaluronic acids were followed a pattern of a non-newtonian fluid. (C) The intensity levels of cellular trail were determined at various hyaluronic acid concentrations. The images of micro motions (arrow heads) of (D) leukocytes, or (E) K562 cells were converted by a motion microscope at 0.5–1.5 Hz. Results are the means ± SE of 6 experiments in each group. *Significantly different from 0.01% hyaluronic acid treated group, P < 0.05