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Fig. 4 | Journal of Biological Engineering

Fig. 4

From: Label-free detection of leukemic myeloblasts in hyaluronic acid

Fig. 4

Role of flow rate and filopodia on the cellular trail. (A) Leukocytes or (B) K562 cells were subjected to the microfluidic device at various flow rates of 25 μm/s, 10 μm/s, or 0 μm/s and cellular trails (arrow heads) were determined. (C) The intensity levels of cellular trails were determined in motion magnified videos. Tumor cells were transfected with CRISPR-CDC42 or CRISPR-control. (D) and (E) Proteins were extracted from each of the cell lines and expression of CDC42, or TUBB were determined using western blotting. (F) Holographic images were recorded by combining laser (λ = 520 nm) that had passed through the cells with up to 30 μm depth of reconstruction. From the 3D images, the outer surfaces of cells were described using optical tomographic microscope. (G) The number and distribution of filopodia on K562 cells. (H-I) Transfected cells were subjected to the microfluidic device and movements were magnified and cellular trails were assessed. Results are the means ± SE of 6 experiments in each group. *Significantly different from cellular trail of leukocytes, P < 0.05. #Significantly different from CRISPR-control transfected cells, P < 0.05

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