Fig. 5

Potential use of motion microscopy for myeloid leukemia detection. (A) Detection of leukemic myeloblasts by cellular trails in PBMCs obtained from patients with myeloid leukemia. Leukemic myeloblasts were immunostained with CD117 antibody using fluorescence microscopy. Leukocytes are indicated by arrows. (B) The intensity level of cellular trails was determined in motion magnified videos. (C) Comparison with the size-based filtration system (screen cell cyto), immunoprecipitation using CD117 antibody, or motion microscope to detect leukemic myeloblasts in human blood samples. (D) Using a non-newtonian fluid, motion microscope is able to detect oscillating movement of leukemic myeloblasts through filopodia under condition of 25 μm/s and 0.5–1.5 Hz. Results are the means ± SE of 6 experiments in each group. *Significantly different from cellular trail of leukocytes, P < 0.05. ^#Significantly different from screen cell cyto method, P < 0.05