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Fig. 1 | Journal of Biological Engineering

Fig. 1

From: Development of a novel defined minimal medium for Gluconobacter oxydans 621H by systematic investigation of metabolic demands

Fig. 1

Cultivation of G. oxydans 621H ΔhsdR pBBR1p264-FDH-ST in a μRAMOS device with 60 g/L fructose and different media. Depicted is the oxygen transfer rate (OTR). Cultivations were performed at 30 °C, 1000 rpm, VL = 500 μL in a 48-well round well microtitre plate at a shaking diameter of 3 mm, initial pH value: 6. Media: Complete Poolman medium as reference (black), Gluconobacter complex medium containing 5 g/L yeast extract, 2.5 g/L MgSO4 ∙ 7H2O, 1 g/L (NH4)2SO4 and 1 g/L KH2PO4 (dark blue), Poolman without salts (light blue), without amino acids (green), without vitamins (orange), without nucleotides (pink), without trace elements (purple). Mean values of at least 4 replicates are shown. The shadows around the curves indicate the standard deviation. For clarity, only every fifth measuring point is marked as a symbol

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