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Fig. 2 | Journal of Biological Engineering

Fig. 2

From: Development of a novel defined minimal medium for Gluconobacter oxydans 621H by systematic investigation of metabolic demands

Fig. 2

Investigation of auxotrophic deficiencies of G. oxydans 621H ΔhsdR pBBR1p264-FDH-ST in Poolman medium regarding the amino acids of group 1 (Asp, Ile, Met, Thr), group 2 (Arg, Pro, His, Glu) and group 3 (Cys, Gly, Ser). Depicted is the oxygen transfer rate (OTR). Cultivation of G. oxydans 621H ΔhsdR pBBR1p264-FDH-ST in a μRAMOS device with 60 g/L fructose, lacking amino acids from group 1 (aspartate, isoleucine, methionine, threonine), group 2 (arginine, glutamine, histidine, proline) and group 3 (cysteine, serine, glycine). Three independent cultivations (A, B and C) were performed at 30 °C, 1000 rpm, VL = 500 μL in a 48-well round well microtitre plate at a shaking diameter of 3 mm, initial pH value: 6. Media: Complete Poolman medium as reference (black) and A without group 1 (light blue), without group 1 with aspartate (purple), without group 1 with isoleucine (green), without group 1 with methionine (orange), without group 1 with threonine (pink), B without group 2 (pink) and without group 2 with glutamate (purple), C without group 3 (green) and without group 3 with glycine (orange). Mean values of at least 6 replicates are shown. The shadows around the curves indicate the standard deviation. For clarity, only every fifth measuring point is marked as a symbol

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