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Fig. 4 | Journal of Biological Engineering

Fig. 4

From: Development of a novel defined minimal medium for Gluconobacter oxydans 621H by systematic investigation of metabolic demands

Fig. 4

Investigation of auxotrophic deficiencies of G. oxydans 621H ΔhsdR pBBR1p264-FDH-ST in Poolman medium regarding the vitamins of group 6 (nicotinic acid, pantothenic acid and p-aminobenzoic acid). Depicted is the oxygen transfer rate (OTR). Cultivation of G. oxydans 621H ΔhsdR pBBR1p264-FDH-ST in a μRAMOS device with 60 g/L fructose, lacking vitamins from group 6 (nicotinic acid, pantothenic acid and p-aminobenzoic acid). Cultivations were performed at 30 °C, 1000 rpm, VL = 500 μL in a 48-well round well microtitre plate at a shaking diameter of 3 mm, initial pH value: 6. Media: Complete Poolman medium as reference (black) and without group 6 (pink), without nicotinic acid (purple), without pantothenic acid (green), without p-aminobenzoic acid (orange) and without vitamins groups 7–9 with group 6 (light blue). Mean values of at least 3 replicates are shown. The shadows around the curves indicate the standard deviation. For cultivation without p-aminobenzoic acid (orange) the mean value of duplicates is shown. For clarity, only every fifth measuring point is marked as a symbol

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