Fig. 5

Cultivation of G. oxydans 621H ΔhsdR pBBR1p264-FDH-ST in a μRAMOS device with 70 g/L fructose and different media. Depicted is A the oxygen transfer rate (OTR) and B the optical density OD₆₀₀ (solid bars) and the product yield g_5KF/g_fructose (hatched bars). Cultivations were performed at 30 °C, 1000 rpm, V_L = 500 μL in a 48-well round well microtitre plate at a shaking diameter of 3 mm, initial pH value: 6. Media: Complete Poolman medium as reference (black), Gluconobacter complex medium containing 5 g/L yeast extract, 2.5 g/L MgSO₄ ∙ 7H₂O, 1 g/L (NH₄)₂SO₄ and 1 g/L KH₂PO₄ (blue), Ameyama minimal medium (pink) and Gluconobacter minimal medium (GMM, green), developed in this work. In A mean values of at least 3 replicates and in B mean values of duplicates are shown. The shadows around the curves indicate the standard deviation. For clarity, only every fifth measuring point is marked as a symbol in A