Development of a novel defined minimal medium for Gluconobacter oxydans 621H by systematic investigation of metabolic demands

Table 1 Composition of Gluconobacter complex medium [33], complete Poolman medium modified by Müller et al. [29, 30, 32], Gluconobacter minimal medium (GMM) and modified Ameyama medium [27]

Ingredients	*Gluconobacter* complex medium [g/L]	Complete Poolman [g/L]	GMM [g/L]	Ameyama [g/L]
Main ingredients
Yeast extract	5
K₂HPO₄				0.5
KH₂PO₄	1	1	1	0.5
NaCl				0.01
(NH₄)₂SO₄	1	1	1
MgSO₄ ∙ 7H₂O	2.5	2.5	2.5	0.2
Amino acids
Alanine		0.24
Arginine		0.125
Aspartate		0.42
Cysteine		0.13
Glutamate		0.5	1.5	6
Glycine		0.175	0.175
Histidine		0.15
Isoleucine		0.21	0.21
Leucine		0.475
Lysine ∙ H₂O		0.495
Methionine		0.125
Phenylalanine		0.275
Proline		0.675
Serine		0.34
Threonine		0.225
Tryptophan		0.05
Tyrosine		0.25
Valine		0.325
Vitamins
Ascorbic acid		0.5
Biotin		0.003
Folic acid		0.001
Nicotinic acid		0.001	0.001	0.0004
Ortoric acid		0.005
P-aminobenzoic acid		0.01	0.01	0.0001
Pantothenic acid		0.001	0.001	0.0004
Pyridoxamine ∙ 2HCl		0.005
Pyridoxine ∙ HCl		0.002
Riboflavin		0.001
Thiamine ∙ HCl		0.001		0.0004
Vitamin B12		0.001
Nucleobases/nucleosides
Adenine		0.01
Cytosine		0.073
Guanine		0.01
Inosine		0.005
Thymidine		0.005
Uracil		0.01
Xanthine		0.01
Trace elements
CaCl₂ ∙ 2H₂O		0.007	0.007
CoSO₄ ∙ 7H₂O		0.005	0.005
CuSO₄ ∙ 5H₂O		0.004	0.004
FeCl₂		0.005	0.005
FeCl₃ ∙ 6H₂O		0.005	0.005
FeSO₄ ∙ 7H₂O				0.001
MnCl₂		0.016	0.016
MnSO₄ ∙ 7H₂O				0.001
(NH₄)₆Mo₇O₂₄ ∙ 4H₂O		0.003	0.003
ZnSO₄ ∙ 7H₂O		0.009	0.009

ISSN: 1754-1611