Fig. 1

MμLAMP for the point-of-care analysis of multiple genes. a Photograph of the 10 microchamber mμLAMP system in a PDMS-glass format. b Structure of the mμLAMP chip. Microchambers were connected to the corresponding thin microchannel via dimension gradient bridges with the whole shape like an octopus. c Direct naked-eye determination of the assay result via the insoluble byproduct magnesium pyrophosphate. d These results were further validated by the green fluorescence induced by the DNA-intercalating dye SYBR green I and (e) the characteristic ladderlike pattern of the LAMP product determined by agarose gel electrophoresis [27]