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Fig. 9 | Journal of Biological Engineering

Fig. 9

From: Microfluidic chip and isothermal amplification technologies for the detection of pathogenic nucleic acid

Fig. 9

Illustration of the entire flow control of the chip. a Initial state of the chip with the RPA mix (red dye) and LAMP mix (blue dye) ,dried primers. b RPA mix was divided into quantitative chambers at 2000 rpm for 30 s. c RPA product was transferred into the amplification chamber as the template for LAMP. d LAMP mix was primed into the siphon valve by the capillary action at 100 rpm for 30 s. e LAMP mix was transferred into the separated sub-volumes at 2000 rpm for 30 s. f LAMP mix was distributed into reaction chambers at 6000 rpm for 60 s [60]

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Journal of Biological Engineering

ISSN: 1754-1611