Fig. 1

Pyroptosis is accompanied by calvarial osteolysis induced by UHMWPE particles. A Representative micro-CT three-dimensional reconstructed images of calvarial (n = 3). Dotted red lines indicate the region of interest. (B&C) BMD and BV/TV in the region of interest shown in (A) were measured. (D&E) H&E staining (D) and TRAP staining (E) of longitudinal sections of calvarial (n = 3), scales bars represent 400 μm. The rightmost pictures designate the larger magnification of the regions shown in inset, scales bars represent 100 μm. F & G Bar charts showing the histological score and integrated optical density (IOD) value of TRAP positive area shown in (D&E). (H) The protein level of caspase-1, IL-1β, and GSDMD detected by western blot analysis. I Bar charts showing the densitometric analysis of western blot shown in (H). J The protein level of IL-1β detected by ELISA in culture supernatant of calvarial tissues. K The relative LDH activity in culture supernatant of calvarial tissues. *P < 0.05, **P < 0.01, ***P < 0.001. P-values were analyzed by two-tailed t tests. All data are representative of two to three independent experiments