Fig. 3
UHMWPE particles induce pyroptosis in osteocytes. A, C, E TUNEL staining (A), caspase-3 IHC staining (C) and H&E staining (E) of longitudinal sections of calvarial (n = 3), scales bars represent 25 μm. B&D Bar charts showing the percentage of TUNEL or caspase-3 positive cells shown in (A&C). (F&G) Bar charts showing the percentage of osteocytes with empty lacunae and the number of osteocytes with empty lacunae per mm2 shown in (E). H caspase-1 IHC staining of longitudinal sections of calvarial (n = 3), scales bars represent 25 μm. I Bar charts showing the percentage of caspase-1 positive cells shown in (H). J&O Western blot analysis of caspase-1, IL-1β, and GSDMD in MLO-Y4 cells induced by different doses of UHMWPE particles for 24 h (J) or by 50 μg/ml UHMWPE particles for different hours (O). K&P Bar charts showing the densitometric analysis of western blot shown in (J&O). L&Q The concentration of IL-1β in culture supernatant of MLO-Y4 cells induced by UHMWPE particles. M&R The relative LDH activity in culture supernatant of MLO-Y4 cells induced by UHMWPE particles. *P < 0.05, ***P < 0.001. P-values were analyzed by one-way ANOVA in (B, D, F, G, K, L, M, P, Q, R), and two-tailed t tests in (I). All data are representative of two to three independent experiments