Fig. 4

Osteocytic pyroptosis facilitates osteoclastic bone resorption. A Schematic of osteoclastic differentiation of BMMs induced by culture supernatant of osteocytes stimulated with UHMWPE particles and/or Ac-YVAD-CMK. Ac represents Ac-YVAD-CMK. B The concentration of IL-1β in culture supernatant of MLO-Y4 cells induced by 50 μg/ml UHMWPE particles and/or Ac-YVAD-CMK. C The relative LDH activity in culture supernatant of MLO-Y4 cells induced by 50 μg/ml UHMWPE particles and/or Ac-YVAD-CMK. D-F The relative mRNA expression of TRAP, NFATc1 and c-Fos in BMMs-derived osteoclasts treated with RANKL and/or culture supernatant of UHMWPE and/or Ac-YVAD-CMK-treated MLO-Y4 cells. G, I, K TRAP staining (G), F-actin ring formation (I) and bone resorption assay (K) of BMMs-derived osteoclasts treated with RANKL and/or culture supernatant of UHMWPE and/or Ac-YVAD-CMK-treated MLO-Y4 cells. H, J, L Bar charts showing the percentage of TRAP positive area, F-actin ring area and bone resorption area shown in (G, I, K). **P < 0.05, ***P < 0.001. P-values were analyzed by one-way ANOVA. All data are representative of two to three independent experiments