Fig. 5

DFO alleviates UHMWPE particle-induced calvarial osteolysis. A Representative micro-CT three-dimensional reconstructed images of calvarial. Dotted red lines indicate the region of interest (n = 3). (B) Bar charts showing the total volume of pore space in calvarial shown in (A). C&D BMD and BV/TV in the region of interest of (A) were measured (n = 3). E&F H&E staining (E) and TRAP staining (F) of longitudinal sections of calvarial (n = 3). Scales bars represent 400 μm. The rightmost pictures designate the larger magnification of the regions shown in inset. Scales bars represent 100 μm. G&H Bar charts showing the histological score and integrated optical density (IOD) value of TRAP positive area shown in (E&F). *P < 0.05, **P < 0.05, ***P < 0.001. P-values were analyzed by one-way ANOVA in (C&D), two-tailed t tests in (B, G&H). All data are representative of two to three independent experiments