Fig. 1
From: High throughput mutagenesis and screening for yeast engineering
Genome-wide screening strategies and collections. a Gene deletion libraries can be constructed using homologous recombination mediation insertion of tagged antibiotic resistance genes (e.g., KanMX4) in place of yeast genes. b Overexpression genomic libraries can be created by placing genomic or cDNA libraries under control of strong (e.g., the inducible GAL1) promoters (GAL1) on a large collection of plasmids and introducing them into yeast cells. c Insertion of a library of sgRNAs into yeast cells that have been engineered to expressed Cas9, dCas9-MXI1, or dCas9-VPR can enable gene deletion, expression inhibition, or transcriptional activation, respectively. The impact of these genetic perturbations can be screened with an appropriate high throughput set up