Fig. 3
From: High throughput mutagenesis and screening for yeast engineering
Schematic representation of high throughput selection schemes in yeast. a Growth based; Strains with improved fitness (in terms of growth rate) naturally outcompete slower growing strains in batch cultures with successive dilutions. b Biosensor; Analyte concentration can stimulate a dose-responsive change in cell growth or reporter gene expression. c Yeast surface display; Proteins of interest are secreted but bound to the yeast cell surface due to their fusion to the Aga2 protein. While anchored to the cell surface, the protein of interest is screened using fluorescent activated cell sorting (shown) or another method (e.g., magnetic bead separations). d Drop-based microfluidics; Diverse populations of single yeast cells, combined with a stabilizer and aqueous drops disseminated in oil, are screened at high throughput based on detector readings