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Fig. 3 | Journal of Biological Engineering

Fig. 3

From: T7Max transcription system

Fig. 3

Cell-free TxTl of GFP from dsDNA circular plasmid template with different promoters. a time course expression of GFP under the classic T7 vs T7Max promoter. b Western Blot analysis of expression of GFP. c RT qPCR cycle (Cq) value quantifying abundance of GFP mRNA. Full, uncropped image of this gel is on Figure S6. d quantification of Western Blots of GFP expression, expressed as unitless relative brightness value. All samples in triplicate, error bars represent S.E.M. Protein product was measured by endpoint measurements after an 8 -hour reaction. All TxTl reactions were incubated at 30°C

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