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Fig. 5 | Journal of Biological Engineering

Fig. 5

From: T7Max transcription system

Fig. 5

Performance of T7Max vs T7 promoter in different template lengths. All green bars: T7 promoter, all blue bars: T7Max promoter. Circular plasmid DNA template expression of proteins with different length of the open reading frame, from 1650 base pairs to 30 base pairs. Each graph shows protein product quantification and corresponding RT qPCR cycle (Cq) value quantifying abundance of mRNA for each protein. All samples in triplicate, error bars represent S.E.M. Protein product was measured by end point measurements after an 8- hour reaction. Luminescence with appropriate luciferase product was used on panels a, d, f. Quantification of appropriate size Western Blot band, expressed as unitless relative brightness value, was used on panels b, e, g and h. Original uncropped gels are on Figure S7. Fluorescence with the arsenic ligand was measured on panel i

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