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Fig. 7 | Journal of Biological Engineering

Fig. 7

From: T7Max transcription system

Fig. 7

Performance of T7Max in different systems. a Cell-free translation reactions based on different organisms. GFP plasmids were prepared for each specific commercial cell-free expression system (except E. Coli, which used the same plasmids as tested earlier, and in house made cell-free expression system). Fluorescence of GFP protein was measured after each reaction, and raw fluorescence was normalized so that classic T7 promoter fluorescence was assigned value 100, and T7Max sample fluorescence was scaled proportionally. All samples are in triplicate, error bars represent standard error. The raw fluorescence data for all normalized data points are on Figure S8, and the method for calculation of error bars (error propagation) is described in Materials and Methods section “Promoter comparison using different extracts”. b Apta-Nucleic Acid Sequence Based Amplification reaction detecting E. Coli gene, aggR. Reactions are identical except for the incorporation of T7Max vs classic T7 promoter. Fluorescence of the broccoli aptamer was measured every 2.5 minutes, excitation: 488 nm and emission: 507 nm, with PMT sensitivity set to Medium for all readouts. All samples were performed in triplicate, and traces represent the average

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