T7Max transcription system

Table 1 List of promoter candidate sequences

Sample ID	Sense strand	Promoter Sequence
1	Uhlenbeck minimal promoter	TAATACGACTCACTATA
2	T7wt promoter	TAATACGACTCACTATAGG
3	T7c62 promoter	TAATACGACTCACAATCGCGGAG
4	Uhlenbeck 600 promoter	TAATACGACTCACTATAGGGATC
5	Uhlenbeck 500 promoter	TAATACGACTCACTATAGGGAGA
6	Uhlenbeck 400 promoter	TAATACGACTCACTATAGGGACT
7	Uhlenbeck 325 promoter	TAATACGACTCACTATAGGGCTC
8	Uhlenbeck 230 promoter	TAATACGACTCACTATAGAGACT
9	Uhlenbeck 117 promoter	TAATACGACTCACTATAGGGAAG
10	Uhlenbeck 73 promoter	TAATACGACTCACTATAGCATCA
11	Uhlenbeck 45 promoter	TAATACGACTCACTATAGGACAT
12	Uhlenbeck 15 promoter	TAATACGACTCACTATACGATCA
13	NASBA promoter	AATTTAATACGACTCACTATAGGGA
14	T7Max Promoter	AATTCTAATACGACTCACTATAGGGA

For most of the promoter sequences we tested here, we named them Uhlenbeck XX, where Uhlenbeck is reference to the original paper the sequences were first reported, and the XX is value reported in Table 5 of reference [11] as picomoles of RNA in test transcription reaction [11]. The NASBA and T7Max primers were based on consensus sequences of promoters known to give robust transcription [14, 15]. While the exact sequence of the promoter that became T7Max has not been, to our knowledge, used in any priori literature, we inferred that sequence from prior work on promoter mutations. In particular, an AT-rich region in the -17 to -22 region and a purine-rich region in the +1-+4 region were important features

ISSN: 1754-1611