Fig. 2
From: A CRISPR interference strategy for gene expression silencing in multiple myeloma cell lines
Rnd3 expression was reduced in RPMI 8226 and JJN3 transduced cells. RPMI 8226 and JJN3 RND3 mRNA expression levels were analyzed by qPCR and calculated as fold change using the 2−ΔΔCt method (A). Representative western blot images of Rnd3 protein expression in RPMI 8226 and JJN3 cells (B). Bands were quantified using ImageJ software and the densitometric values were normalized to GAPDH and plotted in (C). Mean values from 3 independent experiments are plotted and one-way ANOVA and Tuckey’s post-hoc test show differences between groups: *p < 0.05; **p < 0.01, ***p < 0.001, ****p < 0.0001