Fig. 3
From: A CRISPR interference strategy for gene expression silencing in multiple myeloma cell lines
Long-term maintenance of RND3 silencing is observed in RPMI 8226 and JJN3 transduced cells. RNA extracts were obtained from RPMI 8226 and JJN3 cells at different times (1 week, 4 weeks and 8 weeks post-thawing) and RND3 expression was analyzed by RNA sequencing. (A) Rnd3 time course expression in either RPMI and JJN3 cells. Values plotted represent single RNA levels for every cell line at the indicated times. (B) Mean of the values shown in A) grouped for each time and cell line were also plotted to confirm the consistency of the gene silencing along the time. RPMI 8226 knock-down cells show two-fold change expression decrease (p = 0,0009) while JJN3 knock-down show five-fold change expression decrease (p = 0,0002), compared to WT or scramble cells