Table 1 List of primers used to generate the sgRNAs. The necessary tails in the ends for the cloning were highlighted
From: A CRISPR interference strategy for gene expression silencing in multiple myeloma cell lines
Name | Sequence (5’→3’) |
|---|---|
sgRND3 forward #3 | CACCGGAAACGCGGCGCAGACGAGG |
sgRND3 reverse #3 | AAACCCTCGTCTGCGCCGCGTTTCC |
sgRND3 forward #4 | CACCGGGGACTTGGGAGGCGCGGTG |
sgRND3 reverse #4 | AAACCACCGCGCCTCCCAAGTCCCC |
sgRNA Scramble forward | CACCGGGAGGACGATCGTACTCCAG |
sgRNA Scramble reverse | AAACCTGGAGTACGATCGTCCTCCC |