From: A CRISPR interference strategy for gene expression silencing in multiple myeloma cell lines
Name | Function | Description |
---|---|---|
pVSV-G Retroviral vector (pVSV-G) | Envelope | Expresses the G glycoprotein of the vesicular stomatitis virus, necessary for viral entry |
pMDLg-pRRE (pRRE) | Packaging | Includes gag, coding for the virion main structural proteins; pol, responsible for the retrovirus-specific enzymes; and RRE, a binding site for the Rev protein |
pRSV-REV (pREV) | Packaging | Expresses Rev protein, which facilitates export of the RNA from the nucleus |
pLV hU6-sgRND3 #3 hUbC-dCas9-KRAB-T2a-Puro (sgRND3 #3) | Silencing RND3 expression | Expresses all machinery for CRISPRi technique: sgRNA, dCas9 and KRAB |
pLV hU6-sgRND3 #4 hUbC-dCas9-KRAB-T2a-Puro (sgRND3 #4) | Silencing RND3 expression | |
pLV hU6-sgRNA Scramble hUbC-dCas9-KRAB-T2a-Puro (Scramble) | Transduction control |