Fig. 6

Effect of modifying the hot spot residue of nanobody A1F2 on the interaction with AFB1. A Effect of mutated nanobody A1F2 (cartoon and surface) on its interaction with AFB1; CDRs are marked in different colours. B The percentage of the binding of the serially diluted nanobody mutant A1F2 to AFB1 is denoted by the red line solid circles for comparison with the responses of nanobody A1 (black line inverted hollow triangles) and nanobody F2 (black line hollow circles). The dashed lines indicate the nanobody concentration at EC₅₀