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  1. Content type: Methodology

    Precise spatial control and patterning of cells is an important area of research with numerous applications in tissue engineering, as well as advancing an understanding of fundamental cellular processes. Poly ...

    Authors: Mahmoud E Moustafa, Venkat S Gadepalli, Ahmed A Elmak, Woomin Lee, Raj R Rao and Vamsi K Yadavalli

    Citation: Journal of Biological Engineering 2014 8:24

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  3. Content type: Research

    Bioprocesses depend on a number of different operating parameters and temperature is one of the most important ones. Unfortunately, systems for rapid determination of temperature dependent reaction kinetics ar...

    Authors: Martin Kunze, Clemens Lattermann, Sylvia Diederichs, Wolfgang Kroutil and Jochen Büchs

    Citation: Journal of Biological Engineering 2014 8:22

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  4. Content type: Review

    First described in 2004, endometrial stem cells (EnSCs) are adult stem cells isolated from the endometrial tissue. EnSCs comprise of a population of epithelial stem cells, mesenchymal stem cells, and side popu...

    Authors: Javad Verdi, Aaron Tan, Alireza Shoae-Hassani and Alexander M Seifalian

    Citation: Journal of Biological Engineering 2014 8:20

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  5. Content type: Research

    Metal contamination is widespread and results from natural geogenic and constantly increasing anthropogenic sources (mainly mining and extraction activities, electroplating, battery and steel manufacturing or ...

    Authors: Alexandre Duprey, Viviane Chansavang, Franck Frémion, Clémence Gonthier, Yoann Louis, Philippe Lejeune, Fanny Springer, Valérie Desjardin, Agnès Rodrigue and Corinne Dorel

    Citation: Journal of Biological Engineering 2014 8:19

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  6. Content type: Research

    In prior work, a phage engineered with a biofilm-degrading enzyme (dispersin B) cleared artificial, short-term biofilms more fully than the phage lacking the enzyme. An unresolved question is whether the trans...

    Authors: Matthew Schmerer, Ian J Molineux, Dilara Ally, Jabus Tyerman, Nicole Cecchini and James J Bull

    Citation: Journal of Biological Engineering 2014 8:21

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  7. Content type: Research

    Biotechnological screening processes are performed since more than 8 decades in small scale shaken bioreactors like shake flasks or microtiter plates. One of the major issues of such reactors is the sufficient...

    Authors: Clemens Lattermann, Matthias Funke, Sven Hansen, Sylvia Diederichs and Jochen Büchs

    Citation: Journal of Biological Engineering 2014 8:18

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  8. Content type: Research

    Turbulence intensity, or hydromechanical stress, is a parameter that influences a broad range of processes in the fields of chemical engineering and biotechnology. Fermentation processes are often characterize...

    Authors: Andreas Daub, Marina Böhm, Stefanie Delueg, Markus Mühlmann, Gerhard Schneider and Jochen Büchs

    Citation: Journal of Biological Engineering 2014 8:17

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  9. Content type: Research

    In antibody purification processes, the acidic buffer commonly used to elute the bound antibodies during conventional affinity chromatograph, can damage the antibody. Herein we describe the development of seve...

    Authors: Masayuki Tsukamoto, Hideki Watanabe, Ayako Ooishi and Shinya Honda

    Citation: Journal of Biological Engineering 2014 8:15

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  10. Content type: Research

    We discuss fluorescence as a method to detect polycyclic aromatic hydrocarbons and other organic molecules, as well as minerals on the surface of Mars. We present an instrument design that is adapted from the ...

    Authors: Heather D Smith, Christopher P McKay, Andrew G Duncan, Ronald C Sims, Anne J Anderson and Paul R Grossl

    Citation: Journal of Biological Engineering 2014 8:16

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  11. Content type: Research

    The development of novel biomaterials able to control cell activities and direct their fate is warranted for engineering functional bone tissues. Adding bioactive materials can improve new bone formation and b...

    Authors: Ioana-Carmen Brie, Olga Soritau, Noemi Dirzu, Cristian Berce, Adriana Vulpoi, Catalin Popa, Milica Todea, Simion Simon, Maria Perde-Schrepler, Piroska Virag, Otilia Barbos, Gabriela Chereches, Petru Berce and Valentin Cernea

    Citation: Journal of Biological Engineering 2014 8:14

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  12. Content type: Research

    Understanding the fundamental mechanisms underlying the cellular response to topographical surface features will extend our knowledge regarding the regulation of cell functions. Analyzing the cellular response...

    Authors: Mee-Hae Kim, Yoshiko Sawada, Masahito Taya and Masahiro Kino-oka

    Citation: Journal of Biological Engineering 2014 8:13

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  13. Content type: Research

    The computational methods provide condition for investigation related to the process of drug delivery, such as convection and diffusion of drug in extracellular matrices, drug extravasation from microvessels o...

    Authors: Mostafa Sefidgar, M Soltani, Kaamran Raahemifar, Hossein Bazmara, Seyed Mojtaba Mousavi Nayinian and Majid Bazargan

    Citation: Journal of Biological Engineering 2014 8:12

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  14. Content type: Research

    Au/CuS core/shell nanoparticles (NPs) were designed as a new type of transducer agent for photothermal therapy (PTT), with attractive features of easy preparation, low cost and small size for targeting. This p...

    Authors: Ebenezer Addae, Xiuli Dong, Eric McCoy, Chang Yang, Wei Chen and Liju Yang

    Citation: Journal of Biological Engineering 2014 8:11

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  15. Content type: Research

    Osteoarthritis (OA) is a degenerative joint disease affecting approximately 27 million Americans, and even more worldwide. OA is characterized by degeneration of subchondral bone and articular cartilage. In th...

    Authors: Timothy N Snyder, Krishna Madhavan, Miranda Intrator, Ryan C Dregalla and Daewon Park

    Citation: Journal of Biological Engineering 2014 8:10

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    The Erratum to this article has been published in Journal of Biological Engineering 2014 8:27

  16. Content type: Research

    New advances enable long-term organotypic culture of colonic epithelial stem cells that develop into structures known as colonoids. Colonoids represent a primary tissue source acting as a potential starting ma...

    Authors: Asad A Ahmad, Yuli Wang, Adam D Gracz, Christopher E Sims, Scott T Magness and Nancy L Allbritton

    Citation: Journal of Biological Engineering 2014 8:9

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  17. Content type: Research

    The induction of protein synthesis by exogenous delivery of coding synthetic mRNA in desired cells is an auspicious strategy in the fields of basic cell biology, regenerative medicine, treatment of diseases, a...

    Authors: Meltem Avci-Adali, Andreas Behring, Timea Keller, Stefanie Krajewski, Christian Schlensak and Hans Peter Wendel

    Citation: Journal of Biological Engineering 2014 8:8

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  18. Content type: Methodology

    Due to their modular repeat structure, Pumilio/fem-3 mRNA binding factor (PUF) proteins are promising candidates for designer RNA-binding protein (RBP) engineering. To further facilitate the application of the...

    Authors: Zhanar Abil, Carl A Denard and Huimin Zhao

    Citation: Journal of Biological Engineering 2014 8:7

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  20. Content type: Research

    Inducible promoters are widely spread genetic tools for triggering, tuning and optimizing the expression of recombinant genes in engineered biological systems. Most of them are controlled by the addition of a ...

    Authors: Nicolo’ Politi, Lorenzo Pasotti, Susanna Zucca, Michela Casanova, Giuseppina Micoli, Maria Gabriella Cusella De Angelis and Paolo Magni

    Citation: Journal of Biological Engineering 2014 8:5

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  23. Content type: Research

    Lignin derivatives are phenylpropanoid biopolymers derived from pulping and biorefinery processes. The possibility to utilize lignin derivatives from different types of processes in advanced enzyme-catalyzed o...

    Authors: Kristin Johansson, Thomas Gillgren, Sandra Winestrand, Lars Järnström and Leif J Jönsson

    Citation: Journal of Biological Engineering 2014 8:1

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  24. Content type: Letters to the Editor

    Many of the gene regulatory networks used within the field of synthetic biology have extensively employed the AraC and LacI inducible transcription factors. However, there is no Escherichia coli strain that provi...

    Authors: Manjunatha Kogenaru and Sander J Tans

    Citation: Journal of Biological Engineering 2014 8:2

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  26. Content type: Methodology

    Synthetic genomic approaches offer unique opportunities to use powerful yeast and Escherichia coli genetic systems to assemble and modify chromosome-sized molecules before returning the modified DNA to the target...

    Authors: Bogumil J Karas, Bhuvan Molparia, Jelena Jablanovic, Wolfgang J Hermann, Ying-Chi Lin, Christopher L Dupont, Christian Tagwerker, Isaac T Yonemoto, Vladimir N Noskov, Ray-Yuan Chuang, Andrew E Allen, John I Glass, Clyde A Hutchison III, Hamilton O Smith, J Craig Venter and Philip D Weyman

    Citation: Journal of Biological Engineering 2013 7:30

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  27. Content type: Research

    Standardized and well-characterized genetic building blocks are a prerequisite for the convenient and reproducible assembly of novel genetic modules and devices. While numerous standardized parts exist for Escher...

    Authors: Jara Radeck, Korinna Kraft, Julia Bartels, Tamara Cikovic, Franziska Dürr, Jennifer Emenegger, Simon Kelterborn, Christopher Sauer, Georg Fritz, Susanne Gebhard and Thorsten Mascher

    Citation: Journal of Biological Engineering 2013 7:29

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  28. Content type: Research

    Among disposable bioreactor systems, cylindrical orbitally shaken bioreactors show important advantages. They provide a well-defined hydrodynamic flow combined with excellent mixing and oxygen transfer for mam...

    Authors: Wolf Klöckner, Riad Gacem, Tibor Anderlei, Nicole Raven, Stefan Schillberg, Clemens Lattermann and Jochen Büchs

    Citation: Journal of Biological Engineering 2013 7:28

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  29. Content type: Research

    Manufacture of MAT a and MAT α yeast cells is required for crossbreeding, a procedure that permits hybridization and the generation of new heterozygous strains. Crossbreeding also can be performed with a- and α-t...

    Authors: Nobuo Fukuda and Shinya Honda

    Citation: Journal of Biological Engineering 2013 7:27

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  30. Content type: Research

    The use of in silico simulations as a basis for designing artificial biological systems (and experiments to characterize them) is one of the tangible differences between Synthetic Biology and “classical” Genetic ...

    Authors: Brendan M Ryback, Dorett I Odoni, Ruben GA van Heck, Youri van Nuland, Matthijn C Hesselman, Vítor AP Martins dos Santos, Mark WJ van Passel and Floor Hugenholtz

    Citation: Journal of Biological Engineering 2013 7:26

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  32. Content type: Research

    Polyhydroxyalkanoates (PHAs) are a group of biodegradable plastics that are produced by a wide variety of microorganisms, mainly as a storage intermediate for energy and carbon. Polyhydroxybutyrate (PHB) is a ...

    Authors: Asif Rahman, Elisabeth Linton, Alex D Hatch, Ronald C Sims and Charles D Miller

    Citation: Journal of Biological Engineering 2013 7:24

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  33. Content type: Research

    The effects of microchannel diameter in hydroxyapatite (HAp) substrates on osteoblast behavior were investigated in this study. Microchannels of 100, 250 and 500 μm diameter were created on hydroxyapatite disk...

    Authors: Marcello Pilia, Teja Guda, Stefanie M Shiels and Mark R Appleford

    Citation: Journal of Biological Engineering 2013 7:23

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  37. Content type: Research

    Bacillus anthracis is a pathogen that causes life-threatening disease--anthrax. B. anthracis spores are highly resistant to extreme temperatures and harsh chemicals. Inactivation of B. anthracis spores is importa...

    Authors: Xiuli Dong, Yongan Tang, Marvin Wu, Branislav Vlahovic and Liju Yang

    Citation: Journal of Biological Engineering 2013 7:19

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  38. Content type: Research

    Photosynthetic microorganisms that directly channel solar energy to the production of molecular hydrogen are a potential future biofuel system. Building such a system requires installation of a hydrogenase in ...

    Authors: Isaac T Yonemoto, Christopher W Matteri, Thao Amy Nguyen, Hamilton O Smith and Philip D Weyman

    Citation: Journal of Biological Engineering 2013 7:17

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  39. Content type: Research

    The generation of micro- and nano-topography similar to those found in the extra cellular matrix of three-dimensional tissues is one technique used to recapitulate the cell-tissue physiology found in the nativ...

    Authors: Rachel Hatano, Kevin Mercurio, Jesus Isaac Luna, Drew E Glaser, Valerie J Leppert and Kara E McCloskey

    Citation: Journal of Biological Engineering 2013 7:18

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  41. Content type: Research

    We continue our exploration of the large polysaccharide polymer Chitosan as an acute therapy for severe damage to the nervous system. We tested the action of subcutaneously injected nanoparticles (~ 100 – 200 ...

    Authors: Bojun Chen, Debra Bohnert, Richard Ben Borgens and Youngnam Cho

    Citation: Journal of Biological Engineering 2013 7:15

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  43. Content type: Research

    Realizing constructive applications of synthetic biology requires continued development of enabling technologies as well as policies and practices to ensure these technologies remain accessible for research. B...

    Authors: Linda J Kahl and Drew Endy

    Citation: Journal of Biological Engineering 2013 7:13

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  44. Content type: Research

    The chromosomal integration of biological parts in the host genome enables the engineering of plasmid-free stable strains with single-copy insertions of the desired gene networks. Although different integrativ...

    Authors: Susanna Zucca, Lorenzo Pasotti, Nicolò Politi, Maria Gabriella Cusella De Angelis and Paolo Magni

    Citation: Journal of Biological Engineering 2013 7:12

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  45. Content type: Research

    Endothelialization of therapeutic cardiovascular implants is essential for their intravascular hemocompatibility. We previously described a novel nanowell-RGD-nanoparticle ensemble, which when applied to surfa...

    Authors: Katherine E McCracken, Phat L Tran, David J You, Marvin J Slepian and Jeong-Yeol Yoon

    Citation: Journal of Biological Engineering 2013 7:11

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  46. Content type: Research

    Cyanobacteria, prokaryotic cells with oxygenic photosynthesis, are excellent bioengineering targets to convert solar energy into solar fuels. Tremendous genetic engineering approaches and tools have been and s...

    Authors: Hsin-Ho Huang and Peter Lindblad

    Citation: Journal of Biological Engineering 2013 7:10

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  47. Content type: Research

    Embryonic stem cells (ESCs) have been implicated to have tremendous impact in regenerative therapeutics of various diseases, including Type 1 Diabetes. Upon generation of functionally mature ESC derived islet-...

    Authors: Joseph Candiello, Satish S Singh, Keith Task, Prashant N Kumta and Ipsita Banerjee

    Citation: Journal of Biological Engineering 2013 7:9

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  49. Content type: Reviewer acknowledgement

    The editors of the Journal of Biological Engineering thank all our reviewers who have contributed to the journal in volume 5 (2011) and volume 6 (2012).

    Authors: Mark Eiteman

    Citation: Journal of Biological Engineering 2013 7:4

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  50. Content type: Research

    The pluripotency and self renewing properties of human embryonic stem cells (hESC) make them a valuable tool in the fields of developmental biology, pharmacology and regenerative medicine. Therefore, there exi...

    Authors: Nikolai Eroshenko, Rukmani Ramachandran, Vamsi K Yadavalli and Raj R Rao

    Citation: Journal of Biological Engineering 2013 7:7

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  • ISSN: 1754-1611

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Journal of Biological Engineering

ISSN: 1754-1611