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  1. Targeted nucleases have transformed genome editing technology, providing more efficient methods to make targeted changes in mammalian genome. In parallel, there is an increasing demand of Cre-LoxP technology f...

    Authors: Ranjita Sengupta, Amy Mendenhall, Nandita Sarkar, Chandreyee Mukherjee, Amirali Afshari, Joseph Huang and Biao Lu

    Citation: Journal of Biological Engineering 2017 11:45

    Content type: Methodology

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  2. Electronic lab notebooks (ELNs) are better equipped than paper lab notebooks (PLNs) to handle present-day life science and engineering experiments that generate large data sets and require high levels of data ...

    Authors: Erin M. Riley, Holly Z. Hattaway and P. Arthur Felse

    Citation: Journal of Biological Engineering 2017 11:40

    Content type: Research

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  3. Previously we demonstrated that an entire bacterial operon (the PRN operon) is expressible in plants when driven by the Tomato -yellow-leaf-curl-virus (TYLCV) -derived universal vector IL-60.

    Authors: Rita Mozes-Koch, Edna Tanne, Alexandra Brodezki, Ran Yehuda, Ofer Gover, Haim D. Rabinowitch and Ilan Sela

    Citation: Journal of Biological Engineering 2017 11:44

    Content type: Research

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  4. This study examines the effects of adding gelatin to a starch-chitosan composite foam, focusing on the altered structural and biological properties. The compressive modulus of foams containing different gelati...

    Authors: Gregory E. Risser, Brittany L. Banik, Justin L. Brown and Jeffrey M. Catchmark

    Citation: Journal of Biological Engineering 2017 11:43

    Content type: Research

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  5. Locomotor activity of rodents is an important readout to assess well-being and physical health, and is pivotal for behavioral phenotyping. Measuring homecage-activity with standard and cost-effective optical m...

    Authors: Andreas Genewsky, Daniel E. Heinz, Paul M. Kaplick, Kasyoka Kilonzo and Carsten T. Wotjak

    Citation: Journal of Biological Engineering 2017 11:36

    Content type: Methodology

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  6. The accumulated evidence points to the microenvironment as the primary mediator of cellular fate determination. Comprised of parenchymal cells, stromal cells, structural extracellular matrix proteins, and sign...

    Authors: Patrick C. Sachs, Peter A. Mollica and Robert D. Bruno

    Citation: Journal of Biological Engineering 2017 11:34

    Content type: Review

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  7. Oxygen-responsive promoters can be useful for synthetic biology applications, however, information on their characteristics is still limited. Here, we characterized a group of heterologous microaerobic globin ...

    Authors: Alvaro R. Lara, Karim E. Jaén, Juan-Carlos Sigala, Lars Regestein and Jochen Büchs

    Citation: Journal of Biological Engineering 2017 11:39

    Content type: Letters to the Editor

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  8. Aerobic granular sludge has become an attractive alternative to the conventional activated sludge due to its high settling velocity, compact structure, and higher tolerance to toxic substances and adverse cond...

    Authors: Yanan Ren, Fernanda Ferraz, Abbass Jafari Kang and Qiuyan Yuan

    Citation: Journal of Biological Engineering 2017 11:42

    Content type: Research

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  9. This article explains some simple experiments that can be used in undergraduate or graduate physics or biomedical engineering laboratory classes to learn how birdcage volume radiofrequency (RF) coils and magne...

    Authors: Dwight E. Vincent, Tianhao Wang, Thalia A. K. Magyar, Peni I. Jacob, Richard Buist and Melanie Martin

    Citation: Journal of Biological Engineering 2017 11:41

    Content type: Research

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  10. UP elements (upstream element) are DNA sequences upstream of a promoter that interact with the α-subunit of RNA polymerase (RNAP) and can affect transcription by altering the binding RNAP to DNA. However, deta...

    Authors: Qiang Yan and Stephen S. Fong

    Citation: Journal of Biological Engineering 2017 11:33

    Content type: Research

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  11. Human induced pluripotent stem cells (hiPSCs) can form any tissue found in the body, making them attractive for regenerative medicine applications. Seeding hiPSC aggregates into biomaterial scaffolds can contr...

    Authors: Meghan E. Hall, Nima Khadem Mohtaram, Stephanie M. Willerth and Roderick Edwards

    Citation: Journal of Biological Engineering 2017 11:38

    Content type: Research

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  12. The primary objective of Tissue engineering is a regeneration or replacement of tissues or organs damaged by disease, injury, or congenital anomalies. At present, Tissue engineering repairs damaged tissues and...

    Authors: Aleš Gregor, Eva Filová, Martin Novák, Jakub Kronek, Hynek Chlup, Matěj Buzgo, Veronika Blahnová, Věra Lukášová, Martin Bartoš, Alois Nečas and Jan Hošek

    Citation: Journal of Biological Engineering 2017 11:31

    Content type: Research

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  13. The antibiotic methylenomycin A is produced naturally by Streptomyces coelicolor A3(2), a model organism for streptomycetes. This compound is of particular interest to synthetic biologists because all of the a...

    Authors: Jack E. Bowyer, Emmanuel LC. de los Santos, Kathryn M. Styles, Alex Fullwood, Christophe Corre and Declan G. Bates

    Citation: Journal of Biological Engineering 2017 11:30

    Content type: Research

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  14. With additive manufacturing (AM) individual and biocompatible implants can be generated by using suitable materials. The aim of this study was to investigate the biological effects of polylactic acid (PLA) man...

    Authors: Matthias C. Wurm, Tobias Möst, Bastian Bergauer, Dominik Rietzel, Friedrich Wilhelm Neukam, Sandra C. Cifuentes and Cornelius von Wilmowsky

    Citation: Journal of Biological Engineering 2017 11:29

    Content type: Research

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  16. Due to steadily growing population and economic transitions in the more populous countries, renewable sources of energy are needed more than ever. Plant biomass as a raw source of bioenergy and biofuel product...

    Authors: Ahmed Debez, Ikram Belghith, Jan Friesen, Carsten Montzka and Skander Elleuche

    Citation: Journal of Biological Engineering 2017 11:27

    Content type: Review

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  17. In the yeast Saccharomyces cerevisiae, as in every eukaryotic organism, the mRNA 5′-untranslated region (UTR) is important for translation initiation. However, the patterns and mechanisms that determine the effic...

    Authors: Jing Li, Qiang Liang, Wenjiang Song and Mario Andrea Marchisio

    Citation: Journal of Biological Engineering 2017 11:25

    Content type: Research

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  19. The molecular rotor 9-(2-Carboxy-2-cyanovinyl)julolidine (CCVJ) is presumed to have a sensitivity towards velocity or shear which is supposed to result in a change in fluorescence quantum yield. Furthermore, a...

    Authors: Markus J. Schmidt, David Sauter and Thomas Rösgen

    Citation: Journal of Biological Engineering 2017 11:24

    Content type: Research

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  20. The configuration of necrotic areas within the retinal pigmented epithelium is an important element in the progression of age-related macular degeneration (AMD). In the exudative (wet) and non-exudative (dry) ...

    Authors: Qanita Bani Baker, Gregory J. Podgorski, Elizabeth Vargis and Nicholas S. Flann

    Citation: Journal of Biological Engineering 2017 11:26

    Content type: Research

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  21. Poly(γ-glutamic acid) (γ-PGA) is a biopolymer with many useful properties making it applicable for instance in food and skin care industries, in wastewater treatment, in biodegradable plastics or in the pharma...

    Authors: Lena Regestein née Meissner, Julia Arndt, Thomas G. Palmen, Tim Jestel, Hitoshi Mitsunaga, Eiichiro Fukusaki and Jochen Büchs

    Citation: Journal of Biological Engineering 2017 11:23

    Content type: Research

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  22. Recent advances in the expansion and directed pancreatogenic differentiation of human pluripotent stem cells (hPSCs) have intensified efforts to generate functional pancreatic islet cells, especially insulin-s...

    Authors: Elena F. Jacobson and Emmanuel S. Tzanakakis

    Citation: Journal of Biological Engineering 2017 11:21

    Content type: Review

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  23. Escherichia coli is often used for recombinant protein production. The expression of recombinant proteins negatively affects the microbial growth, thus, a balance between protein expre...

    Authors: Tobias Ladner, Martina Mühlmann, Andreas Schulte, Georg Wandrey and Jochen Büchs

    Citation: Journal of Biological Engineering 2017 11:20

    Content type: Methodology

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  25. Synechococcus sp. PCC 7002 (henceforth Synechococcus) is developing into a powerful synthetic biology chassis. In order to streamline the integration of genes into the Synechococcus ch...

    Authors: Anne Ilse Maria Vogel, Rahmi Lale and Martin Frank Hohmann-Marriott

    Citation: Journal of Biological Engineering 2017 11:19

    Content type: Research

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  26. High-throughput methods based on molecular reporters have greatly advanced our knowledge of cell signaling in mammalian cells. However, their ability to monitor various types of cells is markedly limited by th...

    Authors: Zhiwen Zhang, Zachary Stickney, Natalie Duong, Kevin Curley and Biao Lu

    Citation: Journal of Biological Engineering 2017 11:18

    Content type: Research

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  27. Probiotic bacteria are becoming an important tool for improving human health, controlling diseases and enhancing immune responses. The availability of a cost effective cultivation conditions has profound effec...

    Authors: Asma Manzoor, Javed Iqbal Qazi, Ikram ul Haq, Hamid Mukhtar and Akhtar Rasool

    Citation: Journal of Biological Engineering 2017 11:17

    Content type: Research

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  28. Two important and large non-point sources of nitrogen in residential areas that adversely affect water quality are stormwater runoff and effluent from on-site treatment systems. These sources are challenging t...

    Authors: E. V. Lopez-Ponnada, T. J. Lynn, M. Peterson, S. J. Ergas and J. R. Mihelcic

    Citation: Journal of Biological Engineering 2017 11:16

    Content type: Review

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  29. Umbelliferone, also known as 7-hydroxycoumarin, is a phenolic metabolite found in many familiar plants. Its derivatives have been shown to have various pharmacological and chemo-preventive effects on human hea...

    Authors: Luan Luong Chu, Ramesh Prasad Pandey, Haet Nim Lim, Hye Jin Jung, Nguyen Huy Thuan, Tae-Su Kim and Jae Kyung Sohng

    Citation: Journal of Biological Engineering 2017 11:15

    Content type: Research

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  30. Physical scaffolds are useful for supporting cells to form three-dimensional (3D) tissue. However, it is non-trivial to develop a scheme that can robustly guide cells to self-organize into a tissue with the de...

    Authors: Xiaolu Zhu, Shiva Gojgini, Ting-Hsuan Chen, Peng Fei, Siyan Dong, Chih-Ming Ho and Tatiana Segura

    Citation: Journal of Biological Engineering 2017 11:12

    Content type: Research

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  31. Nuclear magnetic resonance (NMR) technique is a powerful analytical tool in determining the presence of bacterial contaminants in complex biological samples. In this paper, a portable NMR-based (pNMR) biosenso...

    Authors: Yilun Luo and Evangelyn C. Alocilja

    Citation: Journal of Biological Engineering 2017 11:14

    Content type: Research

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  32. Nanomedicine has seen a significant increase in research on stimuli-responsive activatable nanoprobes for tumor-specific delivery and diagnosis. The tumor microenvironment has particular characteristics that c...

    Authors: Byunghoon Kang, Aastha Kukreja, Daesub Song, Yong-Min Huh and Seungjoo Haam

    Citation: Journal of Biological Engineering 2017 11:13

    Content type: Review

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  33. Sandwich-type biosensor platforms have drawn lots of attentions due to its superior features, compared to other platforms, in terms of its stable and reproducible responses and easy enhancement in the detectio...

    Authors: Ho Bin Seo and Man Bock Gu

    Citation: Journal of Biological Engineering 2017 11:11

    Content type: Review

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  35. Quantifying gene expression at single cell level is fundamental for the complete characterization of synthetic gene circuits, due to the significant impact of noise and inter-cellular variability on the system...

    Authors: Marilisa Cortesi, Lucia Bandiera, Alice Pasini, Alessandro Bevilacqua, Alessandro Gherardi, Simone Furini and Emanuele Giordano

    Citation: Journal of Biological Engineering 2017 11:8

    Content type: Methodology

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  36. It is challenging to achieve ultrasensitive and selective detection of waterborne pathogens at extremely low levels (i.e., single cell/mL) using conventional methods. Even with molecular methods such as ELISA ...

    Authors: Chao Wang, Foram Madiyar, Chenxu Yu and Jun Li

    Citation: Journal of Biological Engineering 2017 11:9

    Content type: Research

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  37. Deposits of aggregated amyloid-β protein (Aβ) are a pathological hallmark of Alzheimer’s disease (AD). Thus, one therapeutic strategy is to eliminate these deposits by halting Aβ aggregation. While a variety o...

    Authors: Kelly A. Moore, Kayla M. Pate, Deborah D. Soto-Ortega, Samuel Lohse, Nicholas van der Munnik, Mihyun Lim, Kaliah S. Jackson, Venetia D. Lyles, Lemeisha Jones, Nisha Glassgow, Vanessa M. Napumecheno, Shanee Mobley, Mark J. Uline, Rahina Mahtab, Catherine J. Murphy and Melissa A. Moss

    Citation: Journal of Biological Engineering 2017 11:5

    Content type: Research

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  39. During hydrous ethanol production from the sugar refinery industry in Mexico, vinasse is generated. Phenolic compounds and melanoidins contribute to its color and make degradation of the vinasse a difficult ta...

    Authors: Elda España-Gamboa, Teresa Vicent, Xavier Font, Jorge Dominguez-Maldonado, Blondy Canto-Canché and Liliana Alzate-Gaviria

    Citation: Journal of Biological Engineering 2017 11:6

    Content type: Research

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  40. Microbial consortia composed of autotrophic and heterotrophic species abound in nature, yet examples of synthetic communities with mixed metabolism are limited in the laboratory. We previously engineered a mod...

    Authors: Stephanie G. Hays, Leo L. W. Yan, Pamela A. Silver and Daniel C. Ducat

    Citation: Journal of Biological Engineering 2017 11:4

    Content type: Research

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  42. A viable single cell is crucial for studies of single cell biology. In this paper, laser-induced forward transfer (LIFT) was used to isolate individual cell with a closed chamber designed to avoid contaminatio...

    Authors: Yu Deng, Philippe Renaud, Zhongning Guo, Zhigang Huang and Ying Chen

    Citation: Journal of Biological Engineering 2017 11:2

    Content type: Research

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  43. Cellulases are key player in the hydrolyzation of cellulose. Unfortunately, this reaction is slow and a bottleneck in the process chain from biomass to intermediates and biofuels due to low activities of the e...

    Authors: Martina Mühlmann, Martin Kunze, Joaquim Ribeiro, Bertram Geinitz, Christian Lehmann, Ulrich Schwaneberg, Ulrich Commandeur and Jochen Büchs

    Citation: Journal of Biological Engineering 2017 11:1

    Content type: Research

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  45. Biofilm-based microalgal growth was determined as functions of organic chemical loading and water temperature utilizing dairy wastewater from a full-scale dairy farm. The dairy industry is a significant source...

    Authors: Zachary T. Fica and Ronald C. Sims

    Citation: Journal of Biological Engineering 2016 10:18

    Content type: Research

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  46. There have been many trials to visualize smell using various techniques in order to objectively express the smell because information obtained from the sense of smell in human is very subjective. So far, well-...

    Authors: Hwi Jin Ko and Tai Hyun Park

    Citation: Journal of Biological Engineering 2016 10:17

    Content type: Review

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  47. Each cell forever interacts with its extracellular matrix (ECM); a stem cell relies on this interaction to guide differentiation. The stiffness, nanotopography, protein composition, stress and strain inherent ...

    Authors: William J. Hadden and Yu Suk Choi

    Citation: Journal of Biological Engineering 2016 10:16

    Content type: Review

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  48. Cycles of covalent modification are ubiquitous motifs in cellular signalling. Although such signalling cycles are implemented via a highly concise set of chemical reactions, they have been shown to be capable ...

    Authors: Mathias Foo, Rucha Sawlekar and Declan G. Bates

    Citation: Journal of Biological Engineering 2016 10:15

    Content type: Research

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  49. Small-scale micro-bioreactors have become the cultivation vessel of choice during the first steps of bioprocess development. They combine high cultivation throughput with enhanced cost efficiency per cultivati...

    Authors: David Flitsch, Sebastian Krabbe, Tobias Ladner, Mario Beckers, Jana Schilling, Stefan Mahr, Uwe Conrath, Werner K. Schomburg and Jochen Büchs

    Citation: Journal of Biological Engineering 2016 10:14

    Content type: Research

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  50. Alkaline amylase has significant potential for applications in the textile, paper and detergent industries, however, low yield of which cannot meet the requirement of industrial application. In this work, a no...

    Authors: Yingfang Ma, Wei Shen, Xianzhong Chen, Long Liu, Zhemin Zhou, Fei Xu and Haiquan Yang

    Citation: Journal of Biological Engineering 2016 10:13

    Content type: Research

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  • ISSN: 1754-1611 (electronic)

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Journal of Biological Engineering

ISSN: 1754-1611